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tcf lef response element driven luciferase reporter plasmid  (Addgene inc)


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    Structured Review

    Addgene inc tcf lef response element driven luciferase reporter plasmid
    Tcf Lef Response Element Driven Luciferase Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 579 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tcf+lef+response+element+driven+luciferase+reporter+plasmid/M50+Super+8x+TOPFlash+(Plasmid+%2312456)/pm39691387-101-17-34
    Average 96 stars, based on 579 article reviews
    tcf lef response element driven luciferase reporter plasmid - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Phloroglucinol Enhances Anagen Signaling and Alleviates H 2 O 2 -Induced Oxidative Stress in Human Dermal Papilla Cells.
    Article Snippet: Introduction Hair is a distinctive feature of mammals and provides several key advantages for maintaining skin homeostasis, including thermoregulation, sebum production, and protection against ultraviolet radiation [1].. Hair follicles undergo a specific growth cycle comprising three distinct phases (anagen, catagen, and telogen) to maintain tissue homeostasis [2].. The critical components of the hair follicle include hair follicle stem cells (hfSCs) and dermal papilla cells (DPCs), which primarily regulate the hair cycle through intricate signaling interactions [3-5].

    Article Title: Phloroglucinol Enhances Anagen Signaling and Alleviates H 2 O 2 -Induced Oxidative Stress in Human Dermal Papilla Cells
    Article Snippet: .. 293T cells (5 × 10 5 ) were seeded in 6-well plates and incubated at 37°C for 24 h. The cells were transfected with pSV-β-galactosidase plasmid (#E1081; Promega, USA) and TCF/LEF response element-driven luciferase reporter plasmid (#12456; Addgene, USA) using Lipofectamine transfection reagent (#18324012; Invitrogen; Thermo Fisher Scientific). .. The transfected cells were treated with PG (5 or 10 μM) or 5 mM lithium chloride (#L9650; Sigma-Aldrich; Merck KGaA) at 37°C for 24 h. Then, the cells were lysed with passive lysis 5× buffer (#E1941; Promega) for 30 min 4°C.

    Transfection:

    Article Title: Phloroglucinol Enhances Anagen Signaling and Alleviates H 2 O 2 -Induced Oxidative Stress in Human Dermal Papilla Cells.
    Article Snippet: Introduction Hair is a distinctive feature of mammals and provides several key advantages for maintaining skin homeostasis, including thermoregulation, sebum production, and protection against ultraviolet radiation [1].. Hair follicles undergo a specific growth cycle comprising three distinct phases (anagen, catagen, and telogen) to maintain tissue homeostasis [2].. The critical components of the hair follicle include hair follicle stem cells (hfSCs) and dermal papilla cells (DPCs), which primarily regulate the hair cycle through intricate signaling interactions [3-5].

    Article Title: Phloroglucinol Enhances Anagen Signaling and Alleviates H 2 O 2 -Induced Oxidative Stress in Human Dermal Papilla Cells
    Article Snippet: .. 293T cells (5 × 10 5 ) were seeded in 6-well plates and incubated at 37°C for 24 h. The cells were transfected with pSV-β-galactosidase plasmid (#E1081; Promega, USA) and TCF/LEF response element-driven luciferase reporter plasmid (#12456; Addgene, USA) using Lipofectamine transfection reagent (#18324012; Invitrogen; Thermo Fisher Scientific). .. The transfected cells were treated with PG (5 or 10 μM) or 5 mM lithium chloride (#L9650; Sigma-Aldrich; Merck KGaA) at 37°C for 24 h. Then, the cells were lysed with passive lysis 5× buffer (#E1941; Promega) for 30 min 4°C.

    Plasmid Preparation:

    Article Title: Phloroglucinol Enhances Anagen Signaling and Alleviates H 2 O 2 -Induced Oxidative Stress in Human Dermal Papilla Cells.
    Article Snippet: Introduction Hair is a distinctive feature of mammals and provides several key advantages for maintaining skin homeostasis, including thermoregulation, sebum production, and protection against ultraviolet radiation [1].. Hair follicles undergo a specific growth cycle comprising three distinct phases (anagen, catagen, and telogen) to maintain tissue homeostasis [2].. The critical components of the hair follicle include hair follicle stem cells (hfSCs) and dermal papilla cells (DPCs), which primarily regulate the hair cycle through intricate signaling interactions [3-5].

    Article Title: In vitro hair growth-promoting effects of araliadiol via the p38/PPAR-γ signaling pathway in human hair follicle stem cells and dermal papilla cells.
    Article Snippet: .. The pSV-βgalactosidase plasmid (#E1081) was sourced from Promega, whereas the Gli response element-driven luciferase reporter plasmid (#113712), TCF/LEF response element-driven luciferase reporter plasmid (#12456), and VEGF promoter-driven luciferase reporter plasmid (#66128) were acquired from Addgene (MA, United States). .. For transient transfection, 293T cells (2 × 105) were seeded in 6-well plates and incubated at 37°C for 24 h. Subsequently, each reporter plasmid was transfected using the Lipofectamine transfection reagent (#18324,012; Invitrogen; Thermo Fisher Scientific).

    Article Title: Phloroglucinol Enhances Anagen Signaling and Alleviates H 2 O 2 -Induced Oxidative Stress in Human Dermal Papilla Cells
    Article Snippet: .. 293T cells (5 × 10 5 ) were seeded in 6-well plates and incubated at 37°C for 24 h. The cells were transfected with pSV-β-galactosidase plasmid (#E1081; Promega, USA) and TCF/LEF response element-driven luciferase reporter plasmid (#12456; Addgene, USA) using Lipofectamine transfection reagent (#18324012; Invitrogen; Thermo Fisher Scientific). .. The transfected cells were treated with PG (5 or 10 μM) or 5 mM lithium chloride (#L9650; Sigma-Aldrich; Merck KGaA) at 37°C for 24 h. Then, the cells were lysed with passive lysis 5× buffer (#E1941; Promega) for 30 min 4°C.

    Luciferase:

    Article Title: Phloroglucinol Enhances Anagen Signaling and Alleviates H 2 O 2 -Induced Oxidative Stress in Human Dermal Papilla Cells.
    Article Snippet: Introduction Hair is a distinctive feature of mammals and provides several key advantages for maintaining skin homeostasis, including thermoregulation, sebum production, and protection against ultraviolet radiation [1].. Hair follicles undergo a specific growth cycle comprising three distinct phases (anagen, catagen, and telogen) to maintain tissue homeostasis [2].. The critical components of the hair follicle include hair follicle stem cells (hfSCs) and dermal papilla cells (DPCs), which primarily regulate the hair cycle through intricate signaling interactions [3-5].

    Article Title: In vitro hair growth-promoting effects of araliadiol via the p38/PPAR-γ signaling pathway in human hair follicle stem cells and dermal papilla cells.
    Article Snippet: .. The pSV-βgalactosidase plasmid (#E1081) was sourced from Promega, whereas the Gli response element-driven luciferase reporter plasmid (#113712), TCF/LEF response element-driven luciferase reporter plasmid (#12456), and VEGF promoter-driven luciferase reporter plasmid (#66128) were acquired from Addgene (MA, United States). .. For transient transfection, 293T cells (2 × 105) were seeded in 6-well plates and incubated at 37°C for 24 h. Subsequently, each reporter plasmid was transfected using the Lipofectamine transfection reagent (#18324,012; Invitrogen; Thermo Fisher Scientific).

    Article Title: Phloroglucinol Enhances Anagen Signaling and Alleviates H 2 O 2 -Induced Oxidative Stress in Human Dermal Papilla Cells
    Article Snippet: .. 293T cells (5 × 10 5 ) were seeded in 6-well plates and incubated at 37°C for 24 h. The cells were transfected with pSV-β-galactosidase plasmid (#E1081; Promega, USA) and TCF/LEF response element-driven luciferase reporter plasmid (#12456; Addgene, USA) using Lipofectamine transfection reagent (#18324012; Invitrogen; Thermo Fisher Scientific). .. The transfected cells were treated with PG (5 or 10 μM) or 5 mM lithium chloride (#L9650; Sigma-Aldrich; Merck KGaA) at 37°C for 24 h. Then, the cells were lysed with passive lysis 5× buffer (#E1941; Promega) for 30 min 4°C.



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    96
    Addgene inc tcf lef response element driven luciferase reporter plasmid
    Tcf Lef Response Element Driven Luciferase Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tcf+lef+response+element+driven+luciferase+reporter+plasmid/M50+Super+8x+TOPFlash+(Plasmid+%2312456)/pm39691387-101-17-34
    Average 96 stars, based on 1 article reviews
    tcf lef response element driven luciferase reporter plasmid - by Bioz Stars, 2026-11
    96/100 stars
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